饥渴老翁一晚要了我三次,麻豆人妻少妇精品无码区,人妻女友娇妻沉沦系列,97无码精品人妻一区二区

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>CRL-2190 HK-2 人腎近曲小管上皮細(xì)胞.

CRL-2190 HK-2 人腎近曲小管上皮細(xì)胞.

簡(jiǎn)要描述:CRL-2190 HK-2 人腎近曲小管上皮細(xì)胞.,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-15
  • 訪  問  量:1807

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2190 HK-2 人腎近曲小管上皮細(xì)胞.

ATCC® Number:CRL-2190™    Price:$272.00
Designations:HK-2Depositors: RA ZagerBiosafety Level:2 [Cells Contain Papilloma viral DNA sequences ]Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: kidney, cortex Tissue: proximal tubule Cell Type: human papillomavirus 16 (HPV-16) transformedCellular Products:alkaline phosphatase; gamma glutamyltranspeptidase; leucine aminopeptidase; acid phosphatase; cytokeratin; alpha 3, beta 1 integrin; fibronectinPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.CRL-2190 HK-2 人腎近曲小管上皮細(xì)胞.Receptors:epidermal growth factor (EGF), expressedDNA Profile (STR):Amelogenin: X,Y CSF1PO: 13 D13S317: 9 D16S539: 11,12 D5S818: 12 D7S820: 10,11 THO1: 9 TPOX: 8,9 vWA: 17,18Age:*****Gender:maleComments:HK-2 (human kidney 2) is a proximal tubular cell (PTC) line derived from normal kidney. The cells were immortalized by transduction with human papilloma virus 16 (HPV-16) E6/E7 genes. The recombinant retrovirus vector pLXSN 16 E6/E7 containing the HPV-16 E6/E7 genes was used to transfect the ectotropic packaging cell line Psi-2. Virus produced by the Psi-2 cells was used to infect the amphotropic packaging cell line PA317 (see ATCC CRL-9078). Virus produced by the PA317 cells was used to transduce primary PTCs. Although pLXSN 16 E6/E7 also confers resistance to neomycin, selection in G418 was not used to isolate transduced clones. The cell line appears to be derived from a single cell based on Southern and FISH analysis. The E6/E7 genes are present in the HK-2 genome as determined by PCR. The cells retain a phenotype indicative of well differentiated PTCs. They are positive for alkaline phosphatase, gamma glutamyltranspeptidase, leucine aminopeptidase, acid phosphatase, cytokeratin, alpha 3,beta 1 integrin, and fibronectin. The cells are negative for factor VIII related antigen, 6.19 antigen and CALLA endopeptidase. HK-2 cells retain functional characteristics of proximal tubular epithelium such as Na+ dependent / phlorizin sensitive sugar transport and adenylate cyclase responsiveness to parathyroid, but not to antidiuretic hormone. The cells are capable of gluconeogenesis as evidenced by their ability to make and store glycogen. HK-2 cells are anchorage dependent. The cells will not grow in methylcellulose, soft agar or suspension. HK-2 cells can reproduce experimental results obtained with freshly isolated PTCs.Propagation:ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:0.05 mg/ml BPE - provided with the K-SFM kit5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Growth Conditions: Cell growth is dependent on epidermal growth factor. The cells should not be allowed to become confluent. Subculture at 80% of confluence.Subculturing:Protocol:Remove and discard culture medium.Briefly rinse the cell layer with 0.05% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.Incubate cultures at 37°C.


CRL-2190 HK-2 人腎近曲小管上皮細(xì)胞.
















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
asian艳丽的少妇pics| 国产一产二产三精华液区别在哪里| 亚洲熟妇丰满大屁股熟妇图片| 変态奴隷人形ひとみ调教| 小雪被老汉各种姿势玩弄| chinese少妇偷sex国语| 日日噜狠狠天天噜噜噜噜| 奇米777 米奇影视狠狠| 国产av精品一区二区三| 小肚子鼓起按h宫交| 电影巜喂不饱的妻子| 女学生小嫩嫩裸体视频高清WWW| 性xxxfreexxxx国产| 粉嫩小泬无遮挡久久久久久| 人人人妻人人澡人人爽欧美一区| 息与子猛烈交尾在线播放| nba直播在线观看免费| 大sao奶涨奶头痒快来吃| 久久人妻无码毛片A片麻豆| 中文精品久久久久人妻不卡| 国产特级毛片a片www| 亚洲av无码成人精品久久久蜜| 女警乳链乳环蒂环铃铛调教| 国产精品久久久亚洲偷窥女厕| 大乳丰满人妻中文字幕日本| 永不迷路-黑料正能量在线| 男男野外做爰全过程69| 精灵世界的底层训练家| 欧美成人在线视频| 中英文字幕是不是乱码| 国模无码大尺度一区二区三区| 日韩毛片人妻久久蜜桃传媒| 少妇粉嫩小泬喷水视频www| 老扒夜夜春宵第一部| 老汉的性生生活1一7| 特大黑人巨交吊性XXXX| 老汉噗嗤一声整根全进视频| 上司人妻被下属侵犯hd| 免费人成视频在线观看| 人妻被黑人猛烈进入a片| 男女啪啪做爰高潮免费网站|