饥渴老翁一晚要了我三次,麻豆人妻少妇精品无码区,人妻女友娇妻沉沦系列,97无码精品人妻一区二区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>Panc 03.27 人胰腺癌細(xì)胞

Panc 03.27 人胰腺癌細(xì)胞

簡要描述:CRL-2549 Panc 03.27
人胰腺癌細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;
細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!
Panc 03.27 人胰腺癌細(xì)胞

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問  量:1735

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2549 Panc 03.27 人胰腺癌細(xì)胞

ATCC® Number: CRL-2549™ Price: $417.00

Designations: Panc 03.27

Depositors: EM Jaffee

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: pancreas

Disease: adenocarcinoma

Cellular Products: cytokeratins 7 and 18

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-2549 Panc 03.27 人胰腺癌細(xì)胞

Tumorigenic: Yes

Oncogene: K-ras + (wild-type)

Antigen Expression: MHC class I +; MHC class II - [50655]

Blood type A; Rh+

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,11

D13S317: 11,12

D16S539: 12

D5S818: 12,13

D7S820: 8,13

THO1: 6

TPOX: 8,9

vWA: 16,17

Age: 65 years

Gender: female

Ethnicity: White

Comments: Panc 03.27 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.The cells have a reported plating efficiency of 80%.

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 37.5 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X

References: 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602


CRL-2549





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
日本又色又爽又黄的a片吻戏| 国产乱码精品一区二区三区久久 | 米奇7777狠狠狠狠视频| 妻子年轻出轨老了老公要离婚| 超级YIN荡的高中女学生H| 台湾佬中文娱乐网| 色戒完整版无删减158分钟hd| 超h公用妓女精便器系列小说| 人妻巨大乳一二三区| 解开警花的裙子猛烈进入| 日本韩国男男作爱gaywww| 亚洲AV成人无码网天堂| 亚洲精品在线观看| chinese猛1打桩浪小辉| 又粗又长又大真舒服好爽| 蜜桃视频在线观看| 国产精品无码中文在线av| 人色偷偷色av噜噜狠狠99| 蜜桃av噜噜一区二区三区| 午夜成人影院| 精品一区二区免费视频蜜桃网| 熟妇的奶头又大又粗| 公公的淫之手| 丝瓜视频在线观看| 老板含着她的花蒂啃咬高潮的视频 | 美女被强行扒开双腿被桶屁股| 色翁荡息又大又硬又粗又爽小玲| 粉嫩AV久久一区二区三区| 久久精品99国产国产精| 邻居一晚让我高潮3次正常吗 | 顶弄激情出轨h| 亚洲精品第一国产综合野草社区| 精品国产乱码久久久久久口爆| 人人妻人人藻人人爽欧美一区 | 亲妺妺乱的性视频| 国产69精品久久久久| 色一情一区二区三区四区| 不用播放器的av| 亚洲乱码一区二区三区在线观看| 女人下边被添全过视频| 和60岁女人做下面好松|