饥渴老翁一晚要了我三次,麻豆人妻少妇精品无码区,人妻女友娇妻沉沦系列,97无码精品人妻一区二区

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>小鼠正常細(xì)胞>CRL-1772C2C12 小鼠成肌細(xì)胞

C2C12 小鼠成肌細(xì)胞

簡(jiǎn)要描述:CRL-1772 C2C12 小鼠成肌細(xì)胞,
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|;
細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,
提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-1772
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:2198

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1772 C2C12 小鼠成肌細(xì)胞的詳細(xì)介紹

CRL-1772 C2C12 小鼠成肌細(xì)胞

ATCC® Number:CRL-1772™  Price:$256.00
Designations:C2C12

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:myoblast
C2C12 小鼠成肌細(xì)胞


Source:Strain: C3H
Tissue: muscle
Cell Type: myoblast;


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this . AATCC materialnyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-1772

Applications:transfection host (Nucleofection technology from Lonza

Roche FuGENE® Transfection Reagents)



Comments:This is a subclone (produced by H. Blau, et al) of the mouse myoblast cell line established by D. Yaffe and O. Saxel. [22903]

The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins. [22953]

Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic. [23427]

Tested and found negative for ectromelia virus (mousepox).



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C


Subculturing:Protocol: IMPORTANT - DO NOT ALLOW CULTURES TO BECOME CONFLUENT.

Cultures must not be allowed to become confluent as this will deplete the myoblastic population in the culture.

Myotube formation is enhanced when the medium is supplemented with 10% horse serum instead of fetal bovine serum.

  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

    Inoculate at a cell concentration between 1.5 X 10 exp5 and 1.0 X 10 exp6 viable cells/75 cm2.

  6. Incubate cultures at 37°C.




















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
大炕上和岳偷倩| 人妻少妇精品无码专区二区| 东北老妇爽大叫受不了| 年轻又漂亮的后妈2| 欧美激情性做爰免费视频| 亚洲精品国偷拍自产在线观看蜜臀| 男女作爱免费网站| 被夫の上司持久侵犯耻辱在线| 女子全员妊娠计划| 浪荡货老子大吗爽死你h漫画男男| 又大又粗又爽18禁免费看| 老熟妇仑乱视频一区二区| 99精品久久毛片a片| 国产aⅴ激情无码久久久无码| 辽宁老妓女叫床高潮| 蜜桃人妻无码av天堂三区| 污调教贱乳打屁股扒开作文| 免费看男男gay啪啪网站| 中文字幕日本人妻久久久免费| 亚洲精品无码| 久久久无码人妻精品无码| 日本少妇bbw丰满做爰图片| 国产手机拍视频推荐2023| 爆乳邻居肉欲中文字幕樱花动漫| 国精品无码人妻一区二区三区| 国产精品久久久久久爽爽爽床戏| 最近2019年中文字幕无吗| 图书馆h含着粉嫩小奶头h漫画| 欧美性猛交xxxx乱大交3| 9 1免费版下载安装| 久久久久久亚洲av无码专区| 熟女丰满老熟女熟妇| 久久无码人妻一区二区三区| 少妇性按摩无码中文a片| 40厘米黑人与中国女人 | 99久久亚洲精品无码毛片| 蜜桃av色偷偷av老熟女| 精品一区高潮喷吹在线播放| xxxxx性bbbbb欧美| 东北熟妇啪啪流白浆| 夫妻那些事全集免费观看电视剧|